门静脉主支癌栓CD蛋白表达及DNA倍体检测的意义

CD 44 and E-CD Protein Expressions and DNA Ploid Changes in Portal Vein Tumor Thrombosis Following Primary Hepatocellular Carcinoma

目的通过检测内皮钙黏附蛋白(E-CD)和CD44蛋白的表达水平,检测门静脉癌栓中DNA倍体的比例,探讨其在肝癌肝内转移中的作用。方法采用免疫组化方法检测33例门静脉癌栓中E-CD和CD44蛋白的表达,在光镜下对33例门静脉癌栓石蜡标本中肝癌细胞染色体倍数按照超5倍体、2倍体和异倍体进行统计;并与各自原发灶和癌旁组织进行对照。结果①E-CD蛋白的表达:门静脉癌栓、肝癌原发灶、癌旁组织的平均吸光度分别为0.0152±0.0036,0.0527±0.0071和0.1008±0.0063。②CD44蛋白的表达:门静脉癌栓、肝癌原发灶、癌旁组织的平均吸光度分别为0.0159±0.0024,0.0531±0.0092和0.1074±0.0029。③肝癌细胞多倍体检测:肝癌原发灶组超5倍体比例为(25±6)%,而门静脉癌栓组是(58±9)%;各组间和各两组间差异均有统计学意义(P<0.01)。结论门静脉癌栓中E-CD和CD44蛋白表达减少以及DNA超5倍体的增多是肝癌细胞向门静脉转移和门静脉癌栓向肝内转移的重要原因。

Objective To explore CD 44 and E-CD protein expressions and DNA ploid changes in portal vein tumor thrombosis （PVTT） and analyze their roles in intrahepatic metastasis. Methods The protein expressions of E-CD and CD 44 in 33 specimens of PVTT were detected by immunohistochemical method and compared with those in primary tumor and paratumor tissues, as well as the chromosome numbers sorting into pentaploid, diploid and heteroploid were counted under microscope. Results The average light-gaining index of E-CD protein expression in the specimens of PVTT, primary tumor and paratumor tissues was （0. 015 2 ± 0. 003 6）, （0. 052 7 ± 0. 007 1） and （0. 100 8 ± 0. 006 3） respectively and that of CD 44 protein was （0. 015 9 ± 0. 002 4）, （0. 053 1 ± 0. 009 2） and （0. 107 4 ± 0. 002 9） respectively. The rate of hyperpentaploidy cells in the specimens of primary tumor and of PVTT was （25 ± 6）% and （58 ± 9）% respectively （significantly different P〈0. 01）. Conclusion The decreased expressions of E-CD protein and CD44 protein in PVTT increase the possibility of tumor metastasis from PVTT. The increased hyperpentaploidy cells of primary hepatocellular carcinoma directly invade into portal vein to form PVTT.