离子凝胶法制备水杨酸壳聚糖纳米粒

Preparation of chitosan nanoparticles based on an ionic gelation process

目的以壳聚糖为载体材料制备水杨酸壳聚糖纳米粒,并对其制备工艺及体系pH值对药物包封率的影响进行考察,初步探讨壳聚糖纳米粒的载药机制。方法以水杨酸为模型药物,采用离子凝胶法制备壳聚糖纳米粒,以包封率及粒径为指标,考察处方因素对纳米粒制备的影响。结果壳聚糖浓度、体系的pH值、药物质量浓度是影响制备工艺的主要因素;体系的pH值可显著提高壳聚糖纳米粒的包封率。结论药物与壳聚糖之间的离子相互作用较弱,并不是纳米粒载药的主要机制。

Objective To investigate the effects of different methods of elution on the extraction quality of cephalosporin C. Methods Cephalosporin C was absorbed when the fermentation filtrate of cephalosporin C was passed through a resin column of macro-porous adsorption resin XAD-16, and eluted by uniformity elu- tion method and gradient elution method respectively. Results When gradient elution method was adopted, the purity of cephalosporin C in the eluent was 3.23 % higher and the recovery rate of cephalosporin C in the eluent was 2.3 % higher than that of uniformity elution method. Conclusions Gradient elution method is worthy of widely use in cephalosporin C production for its advantages compared with uniformity elution method.