中药吴茱萸中生物碱类成分的HPLC指纹图谱

Fingerprint of alkaloid in Evodia rutaecarpa (Juss.) Benth. by HPLC

目的研究中药吴茱萸中生物碱类成分的HPLC特征指纹图谱。方法应用HPLC法分析吴茱萸药材生物碱类成分色谱图。色谱柱:Agilent Extend C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-水系(水-四氢呋喃-乙酸,体积比48∶1∶0.1);洗脱方式:线性梯度洗脱;柱温:35℃;流速:1 mL.min-1;检测波长:240 nm;进样量:20μL。理论板数按吴茱萸次碱计算不低于3 000。结果10批不同来源的吴茱萸药材所含生物碱类成分均得到很好的分离,获得14个共有特征峰。结论HPLC指纹图谱法能较好地识别吴茱萸药材,为吴茱萸的质量控制提供了方法学依据。

Objective To study the HPLC fingerprints of alkaloid in Evodia rutaecarpa （Juss.） Benth.and establish HPLC standard fingerprints chromatogram. Methods Chromatographic method of alkaloid in Evodia rutaecarpa （Juss.） Benth. was established by using HPLC. Results Fingerprints consisted of 14 common characteristic peaks in 10 samples from different areas. Conclusions This method is accurate, reliable and provides a scientific basis for the control of the quality of Evodia rutaecarpa （Juss.） Benth.