利用羧化纳米磁株抽提微量DNA及荧光定量检测方法的研究

Studies On the Extraction of DNA Using Carboxyl-coated Magnetic Particles and Quantification Using Fluorescent Dye

利用实验室制备的表面修饰有羧基的Fe3O4纳米磁珠对DNA的分离进行了研究,确立了快速、高效、安全、经济的分离纯化方法。利用荧光染料Sybr GreenⅠ对微量DNA进行定量检测,具有灵敏度高、重复性好及操作简单的优点。

Based on the study of extraction of DNA using carboxyl-coated Fe3O4, here we provide a rapid, high efficient, safe and economical method. Using fluorescent dye-Sybr Green I , the quantification of double- strand DNA exhibits the advantages of high sensitivity, good repeatability and convenience.