摘要
初步提纯的马铃薯酷氨酸酶(TYR)固定于海藻酸钠/二氧化硅(ALG/SiO2)凝胶复合物中,并用于含酚废水的处理。在pH=7.0时,固定化酪氨酸酶的比活力为1120 U/g。25℃,以邻苯二酚作底物时,其米氏常数为10.6 mmol/L。相对于游离酶和海藻酸钠固定化酶,本杂化凝胶固定酷氨酸酶具有良好的热稳定性、贮存稳定性及连续使用性。研究表明,本固定化酪氨酸酶有可能实际应用于含酚废水的处理。
An organic-inorganic hybrid gel of alginate/SiO2 （ ALG/SiO2 ） was used for immobilizing the partially purified potato tyrosinase（TYR） for the treatment of phenohc wastewater. The highest activity of immobihzed TYR was 1 120 U/g at pH 7. 0. The Michaelis constant for immobihzed PPO was 10. 6 mmol/L at 25℃ when catechol was used as substrate. This immobilized TYR exhibited a higher thermal stability, storage stability and comtinuous use stabihty than that of free TYR and alginate-immobihzed TYR. This study shows that alginate/SiO2-immobihzed TYR might be a practical tool for removal of phenohc compounds from industrial wastewater.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2007年第10期1169-1172,共4页
Chemical Research and Application
基金
江苏省高校自然科学基金资助项目(03KJD360182)
江苏南通市社会发展计划资助项目(S30017)
关键词
海藻酸钠
酷氨酸酶
正硅酸甲酯
苯酚
废水处理
alginate
tyrosinase
tetramethoxy-silicane
phenol
wastewater treatment
