人细小病毒B19感染与习惯性流产的关系探讨

The relationship between human parvovirus B19 and recurrent pregnancy loss

目的:探讨人细小病毒B 19(Human parvovirus B 19,HPVB19)感染与习惯性流产(Recurrent pregnancy loss,RPL)的关系。方法:建立人细小病毒B 19巢式聚合酶链反应(Nested Polymerase chain reaction,NestedPCR)检测方法,采集习惯性流产孕妇流产组织和正常妊娠人工流产组织,用巢式PCR方法检测流产组织中HPV B 19 DNA。结果:56例RPL孕妇流产组织中HPVB 19 DNA有16例阳性,阳性率28.6%,50例正常妊娠人工流产组织中HPVB19 DNA有2例阳性,阳性率4.0%,2组HPV B19感染率有显著性差异(χ2=12.38,P<0.01)。流产次数与HPV DNA阳性率无显著性关系(χ2=0.03,P>0.05)。结论:RPL孕妇HPV B 19感染率显著高于正常孕妇,HPV B 19感染可能导致习惯性流产的发生。

Objective： To investigate the relationship between recurrent pregnancy loss （RPL） and human parvovirus B19 （I-IPV B19） infection. Methods： 56 abortion tissue specimens from the pregnancy women with RPL and 50 abortion tissue specimens from the normal pregnancy woman were collected. The total DNA were separated from abortion tissue specimens, and HPV B 19 DNA was detected by nested polymerase chain reaction （nested PCR） . Results： 28.6% pregnancy women with RPL were HPV B19 DNA positive and only 4. 0% normal pregnancy women were HPV B19DNA positive. There was no relationship between the number of abortion and the positive ratio of HPV B19 DNA. Conclusion： The rate of HPV B19 infection in pregnancy woman with RPL markedly is higher than that of normal pregnancy woman, it suggests that HPV B 19 perhaps is one of factor of RPL