摘要
以4个人体癌细胞株为模型、利用四甲基偶氮唑盐(methyl thiazolyl blue tetrazolium bromide,MTT)比色法和磺酰罗丹明(SulfoRhodamine B,SRB)染色法对55株来自热带太平洋深海微生物(细菌和霉菌)的培养液的乙酸乙酯抽提物以及菌体的甲醇提取物进行了细胞毒活性筛选,并主要采用分子生物学方法鉴定了该批菌株。结果表明,55株微生物发酵样品共110个提取物中,90%样品表现出细胞毒活性;其中13株微生物的活性较强(提取物有效抑制浓度≤16μg/ml),具有较好的开发应用前景。同时还发现,菌体中检测到的活性菌株数大于发酵液中检测到的活性菌株数,细菌的筛选得率高于霉菌的筛选得率.鉴定结果显示,50株微生物分属于21属、29种,其中13个较高活性菌株来源于8个属。
A total of 110 bio-products, extracted from both the supernatants by ethyl acetate and the precipitated thalli by methanol, derived from the fermented broths of 55 microorganisms including bacteria and molds isolated from deep seas in the tropical Pacific Ocean, were screened for antitumor activity by using 4 human cancer cell lines as the tested models and the techniques of methyl thiazolyl blue tetrazolium bromide (MTT) and sulforhodamine B(SRB) colorimetric assay; while the microbial strains were identified mainly by the molecular biology techniques. Some 90% of the tested 110 extracts appeared antitumor activity and 13 strains of the 55 isolates showed higher inhibitory capacities(effective concerntrations ≤ 16μg/ml), which may imply a possiblility in their further medical application;whereas the percentage of positive extracts was higher from the thalli than that from the supernatants and of actived strains was higher in the bacteria than that in the molds according to this study. The 50 identitied strains belong to 29 species in 21 genera while the 13 higher actived strains distribute in 8 genera. These results probably released a pilot information in the research and development of deep sea microbial resources in China.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第10期81-86,共6页
China Biotechnology
基金
中国大洋矿产研究开发协会资助项目(DY105-04-02-10)
关键词
深海
热带太平洋
抗肿瘤鉴定
细菌
丝状真菌
Deep-sea Tropical Pacific Ocean Antitumor Identification Bacteria Filamentary fungi