板蓝根醇提部位化学成分研究

Study on Chemical Constituents Extracted with Ethanol from Radix Isatidis

目的:从板蓝根中分离化学成分。方法:板蓝根95%乙醇提取物经硅胶吸附,不同溶剂洗脱部分经硅胶柱层析反复分离纯化,测定所得化合物的理化常数和波谱数据,鉴定其化学结构。结果:从氯仿部位分得5个化合物,从正丁醇部位分得1个化合物,分别鉴定为:胆甾醇(Ⅰ)、靛蓝(Ⅱ)、靛玉红(Ⅲ)、对羟基苯甲醛(Ⅳ)、水杨酸(Ⅴ)、落叶松脂素-4,4′-2-O-β-D-二葡萄糖苷(Ⅵ)。结论:化合物Ⅰ、Ⅳ为首次从板蓝根药材中分离,具有一定的参考价值。

OBJECTIVE： To isolate the chemical constituents from Radix isatidis. METHODS： The 95% ethanol extract of Radix Isatidis underwent adsorption by silica gel, and the portion eluted by different solvents was isolated and purified on silica gel column repeatedly. The physico - chemical constants and spectral data of the obtained compounds were determined and their chemical structure was identified. RESULTS： 6 compounds were separated from chloroform and n-Butanol parts of Radix Isatidis and identified as： cholesterol （ Ⅰ ）, indigotin （ Ⅱ ）, indirubin（ Ⅲ ）, p - Hydroxybenzaldehyde （ Ⅳ ）, salicylic acid （ Ⅴ ）, lariclresinol - 4, 4＇ - 2 - O- β - D - glucopyranoside （ Ⅵ）, respectively. CONCLUSION ： For the first time, compound （ Ⅰ ） and compound （Ⅳ） were isolated from Radix Isatidis, this finding serves as reference.