五倍子鞣酸的体外杀精和抑菌作用研究

Spermicidal and Antibacterial Effects in Vitro of Tannic Acid from Chinese Gall

目的:研究五倍子鞣酸的杀精和抑菌作用。方法:按照WHO标准方法,对20例人精液进行体外杀精试验,并观察了五倍子鞣酸对大肠杆菌、金黄色葡萄球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),并将其效果与壬苯醇醚-9(NR-9)作比较。结果:五倍子鞣酸与人精液作用20s最低有效杀精浓度为20mg.mL-1。鞣酸对大肠杆菌(3株)MIC为0.195～0.390mg.mL-1,MBC为0.390～0.780mg.mL-1;对金黄色葡萄球菌(2株)MIC为0.049～0.098mg.mL-1,MBC为0.195～0.390mg.mL-1。而在鞣酸MIC、MBL浓度范围内NP-9无抑菌杀菌作用。结论:五倍子鞣酸具有极强的精液蛋白凝固作用和很好的杀精抑菌效果,可望成为一种安全、有效的阴道杀精抑菌剂,值得进一步研究。

OBJECTIVE： To study the spermicidal and antibacterial effects of tannic acid from Chinese gall. METHODS： Human sperm samples of 20 subjects were taken for spermicidal tests in vitro in accordance with the standard method recom- mended by WTO. The minimum inhibitory concentration and minimum bactericidal concentration （MIC and MBC） of tannic acid from Chinese gall on bacterium coli and staphylococcus aureus were detected. RESULTS： The lowest effective spermicidal concentration of tannic acid at 20s was 20mg·mL^-1.The MIC and MBC of tannic acid against bacterium coli（3 strains） were 0.195- 0. 390mg·mL^-1 and 0. 390- 0.780 mg·mL^-1 respectively, against staphylococcus aureus（2 strains） were 0.049- 0.098mg·mL^-1 and 0.195-0.390mg·mL^-1 respectively. CONCLUSION： Tannic acid from Chinese gall showed potent coagulating effect on spermatines and remarkable antibacterial effect, and which is expected to be an effective and safe vaginal spermicidal and antibacterial agent, further study on which remains to be carried out.