乳增消对乳腺增生大鼠影响的研究

Effects of Ruzengxiao on Rats with Hyperplasia of Mammary Glands

目的:研究乳增消防治大鼠乳腺增生的作用。方法:将切除卵巢的大鼠肌肉注射苯甲酸雌二醇(E2)0.8mg.kg-1,隔天1次,连续90d,在造模20至30d期间,隔天注射1次黄体酮4mg.kg-1,连续5次。在造模30d后,将乳腺增生模型动物随机分组灌胃给药,(0.5%CMC-Na、乳增滴或他莫昔芬)1次.d-1,连续60d;末次给药后24h取样检测各项指标。结果:切除卵巢动物在注射E23mo后,其胸腺指数减小,子宫指数和肾上腺指数均显著增加;其乳腺组织雌激素受体(ER)水平及血清E2水平显著升高,而血清孕酮(P)水平显著降低,E2/睾酮(T)比值显著增加;其乳腺小叶数目增多、间质增生、腺泡内分泌物多,腺泡呈高度扩张状态;其血清催乳素(PRL)水平有所升高,而黄体生成素(LH)及卵泡刺激素(FSH)水平有所降低,但与去势组(卵巢切除动物未注射E2及P等)比较无统计学差异;他莫昔芬和乳增消20g.kg-1在治疗2mo时均有显著对抗E2增加子宫指数和肾上腺指数的作用;乳增消各剂量及他莫昔芬可显著升高乳腺增生大鼠血清睾酮水平,降低E2/T比值,与模型组比较有统计学差异。各药物对乳腺增生大鼠血清LH、FSH、PRL、P及E2等指标均无明显影响。结论:上述研究结果显示,乳增消对模型动物乳腺增生有较好的预防与治疗作用。其作用可能与降低动物血清E2、P及PRL水平,降低E2/T比值有关。

OBJECTIVE： To observe the efficacy of Ruzengxiao in the prevention and cure of hyperplasia of mammary glands in rats. METHODS： To investigate the therapeutic effect of drugs, the spayed rats were injected i.m with benzoic acid E2 0.8mg·kg^-1 qd alt for 90 consecutive days, during the period between 20 and 30 days after modeling, these rats were injected with progesterone 4mg·kg^-1 qd alt for 5 consecutive times; 30d after modeling, the model animals with hyperplasia of mammary glands were randomized to groups to be treated intragastrically with 0.05% CMC- Na,Ruzengxiao or Tomoxi fen qd for 60d consecutively. Samples were taken at 24h after the last time of drug administration for determination of indexes.RESULTS： After treating the model animals for 2months, the following experimental results were obtained： for the spayed animals, after injection with estradiol （E2） for 3mo, their index of thymus decreased, indexes of uterus and adrenal glands increased significantly; levels of estrogen receptor （ER） in mammary tissues and serum E2 increased significantly, while the serum progesterone （P） deceased significantly, ratio of E2/testosterone（T） increased significantly; the numbers of lobules of mammary gland increased, hyperplasia of interstitial tissues appeared, secretion of acini increased, and the acini were highly distended, level of prolactin （PRL） increased, while levels of luteinizing hormone （LH） and follicle stimulating hormone （FSH） decreased, but no statistic difference was noted as compared with castration group （spayed animal without administration with E2 and P etc.）; the E2- induced increase in indexes of uterus and adrenal gland was significantly inhibited after treatment with Tamoxifen or Ruzengxiao 20g · kg^-1 for 2mo; Both Ruzengxlao （at different dosage） and Tomoxifen could significantly increase serum Testosterone level but decrease ratio of E2/T in rats with hyperplasia of mammary glands, showing significant differences as compared with model group. All drugs showed no obvious effect on serum levels of LH, FSH, PRL, P and E2 etc in rats with hyperplasia of mammary glands. CONCLUSION： The above results reveal that Ruzengxiao has satisfactory preventive and curative effects on model rats with hyperplasia of mammary glands. This mechanism might be related to the reduction of serum levels of E2, P and PRL,and the ratio of E2/T.