矿石甲壳类硬质中药索氏提取法的改进

Improvement in Soxhlet Extraction with Ore and Shell Hard Chinese Materia Medica

目的:以龟甲为例,探讨改进矿石甲壳类硬质中药的索氏提取法。方法:在常量索氏提取法的基础上,采取饱和食盐水浴并装置四重盖圈及定时器,实现大量索氏提取法;通过HPLC法、收率和DPPH清除率分析,比较大量索氏提取法、常量索氏提取法和搅拌回流法的优劣。结果:大量索氏提取法和常量索氏提取法没有显著差异,而搅拌回流法较前2种方法效果较差。结论:改进后的大量索氏提取法是一种彻底、高效、方便、经济、健康的方法。

OBJECTIVE ： To discuss the improvement in Soxhlet extraction with ore and shell hard Chinese meterla madica taking Plastrum Testudinis as example. METHODS： On the basis of normal Soxhlet extracter, larger Soxhlet extracter on Plastrum Testudinis was experimented using saturated salt- water-bath, quadruplicity cover circle and timer. Large Soxhlet extracter was compared with normal Soxhlet extracter and stirring - reflux method by HPLC analysis, yield and clearance rate of DPPH. RESULTS： There were no significant difference between normal and larger Soxhlet extracters, but the stirring- reflux method was inferior to these two methods. CONCLUSION： The improved larger Soxhlet extraction is a thorough, high- efficient, convenient, economical and healthy extracting method for ore and shell hard Chinese materia medica.