急性冠状动脉综合征患者外周血单核细胞核因子-κB活性变化的临床探讨

The Clinical Significance of Changes of the Activity of Peripheral Blood Mononuclear Cell NF-κB in Acute Coronary Syndrome.

目的探讨急性冠状动脉综合征(acute coronary syndrome,ACS)患者外周血单核细胞核因子-κB(nuclear fac-tor-kappa B,NF-κB)活性的变化及其临床意见。方法分别采用免疫组织化学染色法和夹心酶联免疫吸附法测定54例ACS,42例稳定型冠心病(SCHD)患者及36例对照组的外周血单核细胞NF-κB活性和血浆可溶性细胞间黏附分子-1(sICAM-1)水平,并分析NF-κB活性与sICAM-1浓度相关性。结果ASC组NF-κB活性和sICAM-1水平显著高于稳定型冠心病组和对照组(P<0.01),而且ACS血NF-κB活性与sICAM-1水平显著正相关。但SCHD组NF-κB活性和sICAM-1水平与对照组比较,差异无显著性意义。结论急性冠状动脉综合征外周血单核细胞核因子-κB活性明显升高,NF-κB是动脉粥样斑块不稳定的标志,并可能通过上调sICAM-1表达而促发ACS。

Objective To investigate the clinical significance of changes of the activity of peripheral blood mononuclear cell NF-κB in acute coronary syndrome （ACS）. Methods Enzyme-linked immunosorbent assay was used to measure the plasma soluble intercellular adhesion molecule-1 （sICAM-1） and immunohistochemical method was used to measure activity of NF-κB in peripheral blood mononuclear cell in 58 patients with ACS, 48 stable coronary heart disease （SCI-ID） and 30 normal control subjects. At the same time linear correlation analysis was used to analyze the correlation between the level of sICAM-1 and the level of activity of NF-κB. Results The level of activity of NF-κB in peripheral blood mononuclear cells and the plasma sICAM-1 cocentration were significantly higher in patients with ACS than in the controls and in patients with SCHD. The level of plasma sICAM-1 was positively correlated with activity of NF-κB in peripheral blood mononuclear cells. There was no significant difference in levels of activity of NF-κB in peripheral blood mononuclear cell and the plasma sICAM-1 concentration between the controls and the patients with SCHD. Condusions The activity of NF-κB in peripheral blood mononuclear cells is significantly elevated in ACS. It is a potential marker of plaque unstability and may induce ACS via up-regulating sICAM-1 expression.