摘要
建立黄花蒿中青蒿素的高效液相色谱测定方法,采用柱前衍生RP-HPLC法测定黄花蒿中青蒿素的含量,采用ZORBAXXDB-C18(4.6mm×150mm,5μm)色谱柱,甲醇-0.01mol/L醋酸钠-醋酸缓冲液(pH5.8,体积比62∶38)为流动相;检测波长:260nm;流速:0.8mL/min;柱温:30℃。对广西、沈阳、北京、郑州、苏州和杭州等不同产地的野生黄花蒿样品、以及同一产地不同采集时间黄花蒿样品进行检测,结果表明不同地区青蒿素含量差异很大,同一地区不同采集时间黄花蒿样品的青蒿素含量也有差异。该法准确可靠、重现性好,能准确地反映青蒿素含量的检测。
The content of artemisimin in Artemidia annua was determined with the method of pre-column reaction to RP-HLPC. The column was ZORBAXXDB-C18 (4. 6 mm × 150 mm, 5 μm), with mobile phase CH3OH-0.01 mol/L NaAC-HAC buffer(pH=5.8,62 : 38) ,flow rate 0.8 mL/min,column temperature at 30 ℃. The results showed that there are great diversity in the content of artemisimin in Artemidiae annua from different places and different harvest time. The method is accurate,sensible and repeatable,and can be used for the quality control of this herbal medicine.
出处
《广西植物》
CAS
CSCD
北大核心
2007年第5期808-810,724,共4页
Guihaia
基金
国家自然科学基金(30660222)
广西科技攻关项目(0663003)
中国科学院农办项目~~