Mel_(1a)受体在无蹼壁虎脊髓中的定位和特征研究

Localization and Characterization of Mel_(1a) Receptor in the Gecko Swinhoniss Spinal Cord

为了进一步了解褪黑素对脊髓不同板层的调节差异,我们运用免疫荧光化学方法,观察了Mel1a受体在无蹼壁虎脊髓中的分布,并对阳性结果进行统计分析.结果显示I-V和X板层的Mel1a受体免疫阳性细胞胞体形体较小,形态多为圆形;VII层的阳性细胞形体较大,形态不规则,呈圆形、三角形或椭圆形三种.统计结果表明,VI层(22.36±2.36个/mm2)、VIII层(24.07±2.05个/mm2)和IX层(30.56±2.87个/mm2)较I-V层(450.25±9.65个/mm2)阳性细胞明显偏少(P<0.05);VII层(476.90±11.26个/mm2)和X(480.64±12.98个/mm2)板层阳性反应细胞密度同I-V板层(450.25±9.65个/mm2)相比没有显著性差异(P>0.05).提示褪黑素通过其受体介导除了对脊髓的感觉传输具有调节作用以外,还可能参与调节爬行动物的运动功能.

In order to learn well about the regulatory function of melatonin to different lamina of spinal cord, we localized and characterized Mella receptor in the spinal cord of Gecko swinhoniss by immunofluorescence. Study demonstrated the presence of Mel1a receptor located mainly in laminae Ⅰ-Ⅴ, lamina Ⅶ and lamina X of the spinal cord. The positive cells were small and mostly round in laminea Ⅰ-Ⅴ and X, while in lamina Ⅶ the positive cells were large and the shapes in them were cycloidal, triangle and elliptical. The statical results showed that the positive cells in lamina Ⅵ （22.36 ± 2.36/mm^2）, lamina Ⅷ （24.07 ± 2.05 /mm^2） and lamina ⅠⅩ （30.56 ± 2.87/mm^2） were evidently lesser than those in laminae Ⅰ-Ⅴ （450.25 ± 9.65/mm^2） （P（0.05） ; The positive cells in lamina Ⅶ （476.90 ± 11.26/mm^2） and lamina X （480.64 ±12.98/mm^2） did not have significant difference with those in laminae Ⅰ-Ⅴ （P〉0.05）. These results indicate that melatonin in spinal cord may play a role in the movement of reptile by its receptors besides the modulatory effect of melatonin on the mechanisms of the spinal cord sensory transmission.