摘要
在甲烷发酵过程中,微量金属元素钴必须以充足的量和生物学有效的形式存在才能为产甲烷菌利用。本研究分别以甲烷产率和辅酶F420为甲烷发酵过程的评价指标,着重考察了钴及其配合物对甲烷发酵的影响。通过比较添加Co2+和配合钴对产甲烷的促进效果,得出在相同摩尔浓度下配合钴更能促进甲烷发酵的结论。不同配体的研究结果表明,在甲烷发酵过程中,丝氨酸是能与Co2+配合、提高Co2+生物可用性的最佳配体。当丝氨酸-钴配合物的添加剂量为2μmol/L时,甲烷产率为313 mL/gCOD,相对空白样提高幅度为60%,此时,辅酶F420的含量由空白样的0.375μmol/gVSS提高到0.516μmol/gVSS,辅酶M的含量由空白样的92.9μmol/gVSS提高到102.6μmol/gVSS。
Cobalt plays an important role in the growth and the metabolism of anaerobic microorganism. To evaluate the effect of cobalt and its complexation, the methane fermentation was investigated by the indictors of methane productivity and the coenzyme F420. The complexation of cobalt was proved to be the positive status of cobalt in methane fermentation. The results obtained in this study indicated that the best complexant was serine, and its addition of 2 μtmol/L led to the highest methane production of 313 mL/gCOD with an increase of coenzyme F420 from 0. 375 μmol/g VSS to 0. 516 μmol/gVSS. The results obtained in this study indicated that the best complexant was serine, and its addition of 2 μmol/L led to the highest methane production of 313 mL/gCOD with an increase of coenzyme F420 from 0. 375 μmol/gVSS to 0. 516 μmol/gVSS.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第5期71-74,共4页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(50678073)
关键词
甲烷发酵
微量元素
钴
配合物
methane fermentation
trace metals
cobalt
complexant
