常见革兰阴性杆菌AmpC酶的检测分析

The detection of common gram-negative bacterial producing AmpCs in our hospital

目的了解常见革兰阴性杆菌、阴沟肠杆菌、铜绿假单胞AmpCs发生率及表型构成,以及产ESBLs的大肠埃希菌、肺炎克雷伯菌AmpC酶的发生率及表型构成。方法采用底物协同-拮抗法(MSSAT)检测大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌、鲍氏不动杆菌、阴沟肠杆菌产AmpCs情况及表型构成。结果产ESBLs的大肠埃希菌和产ESBLs的肺炎克雷伯菌、铜绿假单胞菌、鲍氏不动杆菌、阴沟肠杆菌AmpC酶检出率分别40%、56%、100%、100%、100%。结论产ESBLs大肠埃希菌和肺炎克雷伯菌同时存在AmpC酶的机制,应引起临床和实验室工作者的重视。AmpC酶的耐药表型与菌株的耐药程度相关。

Objective To find out the probability and phenotype of common gram -negative bacterial such as Aeinetobaeter, E. cloacae and Ampcs in PS. aeruginoso and to find out the probability and phenotype of E. eoli and K. pneumonia which producing ESBLs in our hospital. Methods The strain of E. eoli and K. pneumonia Aeinelobaeter, E. cloacae and PS. aeruginoso were detected with muli. substrates synergy - antagonize test （MSSAT）. Results The detection rate of Ampcs in PS. aeruginoso, Aeinelobaeter, E. cloa- cae, E. eoli and K. pneumonia which also producing ESBLs were 100%, 100%, 100%, 40% and 56%, respectively. Conclusion The detection rates of AmpCS are higher in my hospital. E. eoli, K. pneumonia, Aeinelobaeter mainly producing stable derepression, while the E. cloacae, PS. aeruginoso producing highly inducible AmpC.