摘要
以纯化培养的小鼠星形胶质细胞(Astrocyte,AS)为实验材料,采用激光共聚焦钙成像和荧光分光光度计技术,探讨钙激动剂Bay k8644和钙拮抗剂nimodipine对星形胶质细胞胞内钙离子浓度的影响。结果显示,Bay k8644在0.0001、0.001和0.01mmol/L浓度下均可显著增加星形胶质细胞的细胞内钙水平,而nimodipine在0.001、0.01和0.1mmol/L浓度下均可显著降低星形胶质细胞胞内钙水平,并阻止KCl引起的细胞内钙升高。上述结果表明星形胶质细胞对L-型钙通道激动剂和拮抗剂的反应与神经元的反应相似,提示星形胶质细胞胞膜上也存在L-型钙通道。
The laser scanning confocal microscope and fluorescence spectrophotometer were used to investigate the effects of the L-type calcium channel agonist 1,4-dihydro-2,6-dimethyl-5-nitro-4-[2-（trifluoromethyl）phenyl] pyridine-3- carboxylic acid [（±）-Bay k8644] and antagonist nimodipine on intra-cellular calcium concentration （[Ca^2＋]i） of cultured mouse astrocyetes. The results showed that Bay K8644 can significantly increase the [Ca^2＋]i in cultured astrocytes at doses of 0.0001, 0.001 and 0.01 mmol/L, whereas nimodipine can decrease the [Ca^2＋]i at doses of 0.001, 0.01 and 0.1 mmol/L. Nimodipine can also decrease the elevation of [Ca^2＋]i elicited by KCl. Our results suggest that the effects of L-type calcium channel agonist and antagonist on astrocyes are the same as those on neurons, it indicates that astrocytes also have L-type calcium channels.
基金
国家科技部高技术研究发展计划(863计划)(2002AA2Z3115)
