维生素E对阿霉素肾毒性抑制作用的研究

Inhibitory effect of vitamin E on the nephrotoxicity of doxorubicin

目的探讨维生素E(vitamin E,VE)能否抑制阿霉素(doxorubicin,DXR)的肾毒性。方法用DXR,DXR与VE处理肾小球内皮细胞(CRL-1927),通过碱性彗星实验检测DNA损伤程度。结果10μmol/L DXR处理细胞后,彗尾长度明显增加。DXR与VE共同处理细胞后,彗尾长度与DXR处理细胞相比明显变短,并呈时间、浓度依赖性。结论DXR造成了肾小球内皮细胞的DNA损伤,而VE抑制了DXR的肾毒性作用。

Objective To investigate whether vitamin E can inhibit the nephrotoxicity of doxorubicin. Methods After DXR with or without vitamin E treated renal mesangial cell （ CRL-1927 ） , alkaline comet assay was used to detect DNA damage. Results After 10 μmol/L DXR treated cells, the length of comet tails increased obviously. After DXR and VE treated cells together, the length of comet tails decreased in time and concentration-dependent manner when compared to that of control group, Conclusion DXR induced the damage of renal mesangial cell , VE inhibited the nephrotoxicity of doxorubicin.