摘要
采用苯酚-氯仿-异戊醇抽提法提取了采自江西南昌和广州潮州2个地理种群的椭圆食粉螨Aleuro-glyphus ovatus Troupeau,1878基因组DNA。以相应引物对椭圆食粉螨线粒体DNA细胞色素氧化酶I亚基基因(mtDNA COI)进行PCR扩增,直接测序,得到379bp的碱基片断(国际基因库索引号EF527825),其碱基序列A、C、G、T含量分别为71bp(18.73%)、53bp(13.98%)、89bp(23.48%)、166bp(43.80%);江西南昌和广州潮州2个地理种群间的椭圆食粉螨mtDNACOI基因片段完全一致,未发现地理差异。
The genomie DNA ofAleuroglyphus ovatus was extracted by phenol ： Chloroform： isoamyl alcohol, The specimens in the present study were collected from NanChang in JiangXi Province and ChaoZhou in GuangZhou Province. In our research, mitoehondrial eytoehrome oxidase subunit Ⅰ （ CO Ⅰ ） gene fragment of Aleuroglyphus ovatus was successfully amplified and sequenced, 379 hp sequences of mtDNA CO Ⅰ were obtained （accession no. EF527825 in GenBank）. The contents of A,C,G and T were 71 bp （18.73 %） ,53 bp （13.98 %） ,89 bp （23.48 %） ,166 bp （43.80 %）, respectively. There is no difference in the CO Ⅰ gene fragment ofAleuroglyphus ovatus between the two different regions.
出处
《蛛形学报》
2007年第2期79-82,共4页
Acta Arachnologica Sinica
基金
江西省自然科学基金项目(530070)
江西省教育厅科研项目
