HPLC测定桑叶及其炮制品中绿原酸含量

Determination of Chlorogenic Acid in Folium Mori and It’s Processed by HPLC

目的:建立桑叶及其炮制品中绿原酸的含量测定方法,考察桑叶炮制前后绿原酸的含量变化。方法:采用高效液相色谱法,色谱柱为AgilentXDB-C18,流动相为甲醇-0.01mol·L-1磷酸二氢钾(19∶81),pH=3.58,检测波长为328nm,流速为1.0mL·min-1,柱温为35℃。结果:绿原酸进样量在0.25～3.0μg呈良好线性关系,r=0.9999,平均回收率为97.0%,RSD=3.32%(n=5)。桑叶药材与生品中绿原酸含量显著高于蜜炙品,不同地区药材绿原酸含量高低依次为河北>安徽>浙江>江苏>湖南。结论:本方法简单、准确,能有效用于桑叶及其炮制品的质量控制。

Objective： To evaluate the contents of chlorogenic acid between folium mori and it＇s processed, established a HPLC method for the determination of chlorogenic acid. Methods： Chromatographic assay was performed on Agilent XDB -C18 column with methanol-0. 01mol.L^-1 KH2PO4in water solution （19：81） at pH =3.58 as mobile phase, the flow rate was 1.0mL.min^-1 and the detection wavelength was set at 328nm, and the column temperature was 35%. Results： The calibration curve showed linear in the range of 0. 25 -3. 0 μg for chlorogenic acid （r =0. 999 9 ）, and the average＇recovery was 97. 0% （ RSD = 3.32%, n = 5） . It showed that the contents of chlorogenic acid in folium mori and unprocessed were much higher than the honey processed. The chlorogenic acid derived from folium mori coming from different areas has different content （Hebei 〉 Anhui 〉 Zhejiang 〉 Jiangsu 〉 Hunan） . Conclusions： The HPLC method is simple and accurate. It can be used as a method of quality control for Mori and it＇ s processed.