翠云草中酚性成分的分离与鉴定

Isolation and identification of phenolic constituents from Selaginella uncinata(Desv.) Spring

目的研究翠云草[Selaginella uncinata（Desv.） Spring]的化学成分。方法利用硅胶、Sephadex LH-20、ODS柱色谱及反相制备型HPLC等方法进行分离纯化,根据化合物的理化性质及波谱数据鉴定它们的结构。结果与结论从体积分数为60%的乙醇提取液中分离得到7个酚性成分。结构分别鉴定为：阿魏酸棕榈酸-16-醇酯（1）、对羟基桂皮酸（2）、对羟基苯乙酮（3）、香草醛（4）、香草酸（5）、丁香酸（6）、对羟基苯甲酸（7）。化合物1-3为首次从该属植物中分离得到,化合物1-7为首次从该种植物中分离得到。

Aim To study chemical constituents of Selaginella uncinata （Desv.） Spring. Methods The compounds were isolated by means of chromatography on silica gel, Sephadex LH-20 column, ODS column, and reversed-phase Pre-HPLC. Their structures were elucidated by spectroscopic analysis and chemical methods. Results and conclusion Seven phenolic compounds were obtained from the 60 % ethanol extract of Selaginella uncinata （Desv.） Spring and their structures were identified as 16-feruloyloxypalmitie acid（l）, p- hydroxyeinnamie acid（ 2 ）, 1-（ 4-hydroxyphenyl ）-ethanone （3）, vanillin （ 4 ）, vanillie acid （ 5 ）, syringie acid （6）, p-hydroxybenzoic acid（7）. Compounds 1 - 3 were isolated from Selaginella genus for the first time, while compounds 1 - 7 were firstly obtained from Selaginella uncinata （Desv.） Spring.