复合扩增18个基因座的初步研究

A study on co-amplification of the 18 CODIS STR Loci

目的建立五色荧光18个基因座的复合扩增检验体系。方法设计、合成引物,通过调整引物浓度和复合扩增条件,建立起五色荧光复合扩增体系,包含Amelogen、D6S1043、D21S11、D7S820、CSF1PO、D2S1338、D3S1358、D13S317、D8S1179、D16S539、PentaE、TH01、TPOX、PentaD共计18个基因座。结果该复合扩增系统检验结果稳定,分型准确。结论五色荧光18个基因座复合扩增系统构建成功。

A study of 18-1oci multiplex was carried out on the co-amplification of seventeen STR loci plus the gender-determining locus Amelogen in a single amplification/detection of all CODIS （Combined DNA Index System） STR loci （CSF1PO, D3S1358,DSS818,D7S820,D8S1179,D13S317,D16S539,D18S51,D21S11,FGA,TH01,TPOX,and vWA） as well as four widelyaccepted STRs （D6S1043, PentaE,PentaD and D2S1338）. By using five-dye fragment analysis technology and previously optimized DNATyperTM15 kit primer sequences, the results showed the multiplex sucessfully co-amplify and simultaneously detect all 18 loci and the multiplex could be a useful tool for forensic evidence testing.