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牙齿的DNA提取及STR分型研究 被引量：42

DNA extraction and STR genotyping from teeth
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摘要 目的建立有效的牙齿DNA提取方法。方法使用物理及化学方法去除牙齿表面污染物,经脱钙、裂解、纯化从牙粉中提取DNA进行STR分型。结果对96例牙齿检材进行DNA检验,获得STR分型的有94例,在查找尸源的案件中发挥了重要作用。结论本方法操作简单快速,能够显著提高牙齿DNA检验的成功率。 Objective To establish an efficient DNA extraction method for individual identification from teeth samples. Method The teeth DNA was extracted using cleansing, pulverizing , de-calcification, lysis and purification , followed by STR typing. Result 94 DNA profiles were successfully obtained from 96 teeth samples, Conclusion This new approach can increase the success rate of teeth DNA typing by streamlining extraction protocol.
作者 凃政 刘志芳 陈松 李万水
机构地区 公安部物证鉴定中心
出处 《刑事技术》 2007年第5期9-11,共3页 Forensic Science and Technology
关键词 牙齿 DNA提取 复合扩增 STR分型 tooth DNA extraction multiplex amplification STR typing
分类号 D919.2 [医药卫生—法医学]
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参考文献5

  • 1[1]Kemp B M,Smith D G.Use of bleach to eliminate contaminating DNA from the surface of bones and teeth[J].Forensic Sci Int,2005,154:53-61.
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  • 3杨周岐,张虎勤,张金,赵文明.古人类骨骼DNA降解影响因素分析[J].第四军医大学学报,2006,27(1):90-92. 被引量：18
  • 4[5]Imaizumia K,Noguchi K.DNA typing of bone specimens the potential use of the profiler test as a tool for bone identification[J].Legal Medicine,2005,7:31-41.
  • 5[6]Gill P,Whitaker J,Flaxman C,et al.An inverstigation of the rigor of interpretation rules for STRs derived from less than 100pg of DNA[J].Forensic Sci Int,2000,112:17-40.

二级参考文献20

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共引文献17

同被引文献190

引证文献42

二级引证文献69

刑事技术
2007年 第5期

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