大鼠重症急性胰腺炎时IL-6、TNFα-和微循环变化的实验研究

Changes of blood IL-6,TNF-α and microcirculation in rats with severe acute pancreatitis

目的:探讨大鼠重症急性胰腺炎时IL-6、TNF-α和微循环的变化。方法:将Wistar大鼠40只随机分为2组,假手术组(n=20)开腹后仅轻揉胰腺及十二指肠。SAP组(n=20)向大鼠胰管注射牛磺胆酸钠建立SAP动物模型。分别测定2组动物实验开始时和开始后30、60、90、120、150和180min时的胰腺区血流量,以起始数据为基数,计算各测定值的百分数;于实验前和实验后2、4、6、12h经颈静脉取血,采用双抗体夹心ELISA法测定血清中白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平。术后12h处死大鼠,取胰腺组织行病理检查。结果:假手术组实验后180min内血流量无明显改变。SAP组胰头部及胰体尾部血流量均在30min时明显下降,以后呈逐渐下降趋势,与假手术组比较有显著性差异(P<0.01)。术前假手术组和SAP组IL-6、TNF-α水平差异无显著意义(P>0.05)。术后IL-6、TNF-a水平均升高,两组差异有显著性(P<0.01)。胰腺病理组织学评分假手术组明显低于SAP组,差异有显著性(P<0.01)。结论:SAP时胰腺微区血流量明显下降,出现微循环障碍;且血IL-6、TNF-α水平明显升高是加重SAP病情的损伤性因子。

Objective： To study the changes of blood IL-6, TNF-α and microcirculation in severe acute pancreatitis （SAP）. Methods： Fourty Wistar rats were divided into 2 groups .-sham operation group （group I） and SAP group （group Ⅱ）. An animal model of acute pancreatitis was established by injecting sodium taurocholate into the pancreatic duct. The pancreatic microcirlution was studied and the value at was recorded at 0,30,60,90,120,150,180 min. Blood samples were taken from the jugular vein before the experiment and at 2,4,6,12 hours after the experiment. The IL-6 and TNF-α were detected by ELISA technique. The pancreas was examined histologically 12 hours after the treatment. Results： The results show that the microflow of sham operation group was not changed and with the development of SAP, there was a progressive decrease in perfusion of pancreas. The serum levels of IL-6 and TNF-α of the sham operation group were lower than those of SAP group, the difference was significant（P〈0.01）. The grade of pancreatic histopathology of the sham operation was obviously lower than that of SAP group （ P 〈0. 01）. Conclusions：The cytokines （TNF-α,IL-6） play an important role in the development of acute pancreatitis and they can aggravate it.