Th1/Th2类细胞因子转换对小鼠心脏移植物存活的影响

Th1/Th2 cytokine profiles and their relationship to the survival time of cardiac allografts in mice

目的探讨Th1/Th2类细胞因子转换对小鼠心脏移植物存活时间的影响。方法采用小鼠腹部心脏移植模型,分为同种异体移植组(A组)、同种异体移植+免疫抑制处理组(B组)和同系移植组(C组),每组20对。观察移植物存活时间、供心病理改变、受鼠脾和供心内IFN-γ,IL-2,IL-4及IL-10 mRNA的表达水平。结果A组及B组移植物平均存活时间分别为(7.8±0.77)d和(14.80±1.01)d,C组移植物存活均超过28d;3组间差异均有显著性(P<0.05)。A组与B组、C组比较,移植物的心肌细胞变性坏死严重,并有大量炎性细胞浸润。A组受鼠脾脏及供心内IFN-γ和IL-10 mRNA表达比其余两组明显增强;3组移植心组织IL-2及IL-4 mRNA均无表达;A组脾脏IL-2 mRNA表达最强;B组脾脏IL-4 mRNA表达明显强于其余两组。结论Th1/Th2转换在延长移植物存活过程中起重要作用;IL-10也参与移植物排斥反应过程。

Objective To study the relationship between Th1/Th2 cytokine profiles and the survival time of cardiac allografts in mice. Methods The ventral heterotopie cardiac transplantation models were divided into three groups： rejection group, treated group, isograft group, each group with 20 recipients. Mean survival time（MST）, pathologic histological changes, the mRNA expression of IFN-γ, IL-2, IL-4 and IL-10 were measured. Results MST of heart allografts in rejection and treated group was （ 7. 8± 0. 77 ） d, and （14.80±1.01 ）d respectively. The survival time of grafts in isograft group were all more than 28d. The difference among the three groups was significant. In rejection group, the number of infiltrating cells was much more than that in the other groups, and also, the extent of pathologic histological changes was more severe. The mRNA expression of IFN-γ and IL-10 in graft and spleen in rejection group was much stronger than that in the others. There was no obvious mRNA expression of IL-2 and IL-4 in grafts of all three groups. The mRNA expression of IL-2 in the spleens of rejection group was the strongest, while the mRNA expression of IL-4 in the spleens of treated group was the strongest. Conclusions The dynamic equilibrium of Th1/Th2 cytokines plays an important role in the survival time prolongation of cardiac allografts in mice. IL-10 can also participate in the process of graft rejection.