当归、丹参、钙剂对庆大霉素肾损伤防护作用的实验研究

Protective Effects of Angelicae Sinensis, Redix Salviae Miltiorrhizae and Calcium Solution on Renal Damage Cuased by Gentamicin in Rats

本实验给大鼠注射庆大霉素100mg/kg2小时后,用显微分光光度计定量测定肾皮质Na-K-ATP酶的活性,发现其明显下降;而在每只静脉注射一定剂量的当归、丹参注射液或每天口服10％葡萄糖酸钙5天后,则可防止庆大霉素造成该酶活性的下降。本实验提示:在临床中使用当归、丹参或钙剂,可能有助于减轻庆大霉素的肾损伤作用。

We studied the protective effects of Angelica sinensis (As), Dansen and calsium solution against the decline of the renal cortical Na-K-ATPase, ACpase and AKPase aetivities caused by gentamicin in rats using a computer commu- nicated microspectrophotometer. The Na-K-ATPase activity was significantly declined in the gentamicin group as compared with that of the control group (28.16±15.6 vs. 106.58± 43.40 au, p<0.001). In the As, Calcium and Dansen groups, it was mark- edly higher than that in the gentamicin group, showing no significant differen- ce from that in the control group (being 86.95±24.49 and 83.13±17.64 and 73.94±16.36 au. respectively). The ACPase and AKPase activities in all experimental groups exhibited no significant difference from that of the control group. It is concluded that 1) acute exposure to 100mg/kg B.W. of genta- micin results in a decline of renal cortical Na-K-ATPase activity; 2) As, Dansen and Calcium solution prevent the decline of the enzyme activity caused by gentamicin; 3) since the inhibition of the enzyme activity is an early and primary event in the pathogenesis of gentamicin nephrotoxicity, As, Calcium solution and Dansen may be beneficial to patients with gentamicin nephrotoxicity when used in the clinic.