摘要
Recently bacterial cells have become attractive biological templates for the fabrication of metal nano- structures or nanomaterials due to their inherent small size, various standard geometrical shapes and abundant source. In this paper, nickel-coated bacterial cells (gram-negative bacteria of Escherichia coli) were fabricated via electroless chemical plating. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) characterization results reveal evident morphological difference between bacterial cells before and after deposition with nickel. The bare cells with smooth surface presented transverse outspreading effect at mica surface. Great changes took place in surface roughness for those bacterial cells after metallization. A large number of nickel nanoparticles were observed to be equably distributed at bacterial surface after activation and subsequent metallization. Furthermore, ultra thin section analytic results validated the presence and uniformity of thin nickel coating at bacte- rial surface after metallization.
Recently bacterial cells have become attractive biological templates for the fabrication of metal nanostructures or nanomaterials due to their inherent small size, various standard geometrical shapes and abundant source. In this paper, nickel-coated bacterial cells （gram-negative bacteria of Escherichia coll were fabricated via electroless chemical plating. Atomic force microscopy （AFM） and transmission electron microscopy （TEM） characterization results reveal evident morphological difference between bacterial cells before and after deposition with nickel. The bare cells with smooth surface presented transverse outspreading effect at mica surface. Great changes took place in surface roughness for those bacterial cells after metallization. A large number of nickel nanoparticles were observed to be equably distributed at bacterial surface after activation and subsequent metallizaUon. Furthermore, ultra thin section analytic results validated the presence and uniformity of thin nickel coating at bacterial surface after metallization.
基金
Supported by the National Natural Science Foundation of China (Grant Nos. 60171005, 60371027, 60121101, 20573019 and 90406023)
Open Project Foun-dation of Laboratory of Solid State Microstructures of Nanjing University
关键词
原子粒显微镜
电子显微镜
金属化
细菌细胞
biotemplate, atomic force microscopy （AFM）, transmission electron microscopy （TEM）, metallization, bacterial cell
