成骨细胞分泌白细胞介素-6及其调控在骨质疏松症中的作用

Production of IL 6 by osteoblasts and its regulation: a preliminary study on mechanism of primary osteoporosis

目的研究成骨细胞分泌白细胞介素－６（ＩＬ－６）在骨吸收代谢中的作用。方法从新生大鼠颅骨分离出成骨细胞，采用杂交瘤细胞系Ｂ９细胞增殖方法检测细胞ＩＬ－６的分泌量，并研究白细胞介素－１（ＩＬ－１）、肿瘤坏死因子（ＴＮＦ）、细菌脂多糖（ＬＰＳ）等因素对其活性的影响。结果新生鼠颅骨成骨细胞能自发分泌ＩＬ－６，该细胞的上清液能促进ＩＬ－６依赖的杂交瘤细胞系Ｂ９细胞增殖，其促增殖作用与细胞浓度有关，并可被抗ＩＬ－６抗体所抑制；在未加入单克隆抗体８的实验组内，ＩＬ－６的平均分泌值为３５．１±１２．０ｋＵ／Ｌ，明显高于加入单克隆抗体８组（４．３±６．０ｋＵ／Ｌ）。且当ＩＬ－１量从１ｋＵ／Ｌ增至１００００ｋＵ／Ｌ时，成骨细胞分泌量明显提高（最大３１０ｋＵ／Ｌ）。ＴＮＦ、ＬＰＳ也促进大鼠成骨细胞分泌ＩＬ－６。结论绝经期后雌激素水平下降引起的骨质疏松，可能与成骨细胞及其分泌ＩＬ－６的作用有关。

Objective To demonstrate the roles of osteoblasts and IL 6 in the metabolism of bone resorption. Methods Osteoblasts were isolated from newborn rat calvaria and cultured. The activity of IL 6 produced by the cells was measured with the method of hydrima clone B9 growth and the influence of IL 1,TNF,LPS on activity of IL 6 was studied. Results IL 6 was spontaneously produced by osteoblasts from newborn rat calvaria (35 1±12 0kU/L). The cell medium could promote the growth of IL 6 dependent hydrima clone B9 and that effect was associated with cell concentration and inhibited by anti IL 6 antibody (4 3±6 0kU/L). IL 1 in doses from 1kU/L to 10 000kU/L stimulated dose dependent increase of IL 6 and similar increase occurred after stimulation with TNF and LPS. Conclusions Bone loss after reduction of estrogen in postmenopausal patients may be associated with production of IL 6 by osteoblasts.