乌苯美司对白血病细胞生长及 P388 淋巴结转移的抑制作用

Inhibitory effect of ubenimex on growth of human leukemia cells and lymph node metastasis of leukemia P388

目的：观察乌苯美司对白血病细胞生长及淋巴结转移的抑制作用。方法：将ＨＬ－６０，Ｋ５６２，ＭＴ－１，ＭＴ－２，ＭＯＬＴ－４和Ｒａｊｉ白血病细胞分别与乌苯美司培育，计数存活细胞，测定ＭＴ－１和ＭＴ－２的［３Ｈ］ＴｄＲ参入量及Ｐ３８８细胞淋巴结转移。结果：乌苯美司４５００ｍｇ／Ｌ使ＨＬ－６０，ＭＴ－１，ＭＴ－２和Ｒａｊｉ细胞存活数明显减少（Ｐ＜０．０１），０．１，１，１０，１００和１０００ｍｇ／Ｌ可明显抑制ＭＴ－１，ＭＴ－２细胞摄入［３Ｈ］ＴｄＲ（Ｐ＜０．０１）；小鼠每天ｉｐ乌苯美司１，３，１０，３０μｇ／鼠×１１ｄ，转移至淋巴结的Ｐ３８８细胞数明显减少。结论：乌苯美司能抑制人白血病细胞生长及ＭＴ－１，ＭＴ－２细胞的ＤＮＡ合成，有效抑制Ｐ３８８细胞的淋巴结转移。

AIM: To observe the inhibitory effect of ubenimex (Ube) on human leukemia cells and lymph node metastasis. METHODS: Human leukemia cells (HL-60, K562, MT-1, MT-2, MOLT-4, and Raji) were cultured with Ube. The viable cells, TdR uptake of MT-1 and MT-2 cells, and lymph node metastasis of leukemia P388 were determined. RESULTS: Ube 4500 mg/L decreased the number of viable HL-60, MT-1, MT-2 and Raji (P<0.01). TdR uptake of MT-1 and MT-2 cells were inhibited at Ube 0.1, 1, 10, 100 and 1000 mg/L(P<0.01). Mice were injected ip Ube 1, 3, 10 and 30 μg/mouse for 11 d, the numble of lymph node metastasis of leukemia P388 was diminished. CONCLUSION: Ube inhibited the growth of human leukemia cells and the DNA synthesis of MT-1 and MT-2 cells. The lymph node metastasis of leukemia P388 were efficiently suppressed.