脾切除及大肠杆菌内毒素攻击对大鼠血浆内毒素、TNF和NO水平的影响

The effect of splenectomy and Escherichia coli endotoxin insult on the plasma endotoxin, TNF and NO levels in rats

本实验动态观察了大鼠脾切除及粗制大肠杆菌内毒素攻击后血浆内毒素、肿瘤坏死因子（ＴＮＦ）和ＮＯ－２／ＮＯ－３水平的变化。结果发现：脾切除组较假手术组血浆上述因子均明显升高（Ｐ＜０．０５或Ｐ＜０．０１），且和单纯腹腔注射大肠杆菌内毒素后所引起的改变非常类似。注射内毒素后，切脾前后血浆上述因子无明显差异，但均处在较高水平上，秩相关分析表明：血浆ＮＯ－２／ＮＯ－３与内毒素及ＴＮＦ水平有一定相关性（ｒｓ分别为０４２２及０７２１，Ｐ均＜００１），其中与ＴＮＦ高度相关。作者认为：脾切除后机体免疫力下降，可能导致肠道细菌或毒素移位，诱导产生ＴＮＦ等细胞因子，并协同激活体内Ｌａｒｇ：ＮＯ通路。这也可能是脾切除后凶险性感染（ＯＰＳＩ）发生机制之一。

The present study observed the significance of the serial changes of plasma NO - 2/NO - 3, tumor necrosis factor (TNF) and endotoxin concentration levels after splenectomy and sepsis. The data showed that the concentration of plasma NO - 2/NO - 3 was significantly increased at all time points after splenectomy ( P <0.05, vs. the sham). When injected with endotoxin, there was no significant difference between the splenectomy group and the sham operation group, but both were in the high levels. The same changes had been seen in the plasma TNF and endotoxin concentrations. There was a significant corelationship between the NO - 2/NO - 3 and TNF concentration ( r s =0.721, P <0.001) or endotoxin levels ( r s =0.422, P <0.01). These results indicate that endotoxin and TNF may induce nitric oxide synthesis after splenectomy. It may be one of the pathogenetic mechanisms of overwhelming postsplenectomy infections (OPSI).