气相色谱用于肠通透性监测的研究

Measurement of urinary content of lactulose and mannitol by gas chromatography as an index of permeability of the gut

作者采用国产气相色谱及９２０２微机数据处理系统，对临床患者和实验动物肠通透性改变进行监测。该方法以甘露醇（Ｍ）和乳果糖（Ｌ）为探针，用气相色谱检测尿中糖分泌率及Ｌ／Ｍ比值。结果显示，测定标准品Ｍ和Ｌ随进样量增加而呈线性改变，与国外学者报道一致。比较不同浓度进口与国产乳果糖，证明二者在研究肠通透性方面高度相关（ｒ＝０．９９）。检测急性胰腺炎并发感染动物的尿标本，发现乳果糖大量排出，Ｌ／Ｍ值明显增加。作者认为，气相色谱为一行之有效的方法用于肠通透性监测，有助于临床上对内源性感染及脓毒症的早期诊断。

We established lactulose mannitol(L M) measurement method by gas chromatography and 9202 computer data processing system to test intestinal permeability.The urine output of L M was in linear correlation to its sample concentration within working range. In an animal model of acute pancreatitis, lactulos secretion increased in urine, together with increased L/M ratio.The measurement of lactulose mannitol intestinal permeability by our method might serve as a predictor for early diagnosis of endogenous infection and sepsis.