睾丸畸胎瘤核仁组成区嗜银蛋白定量研究

Nucleolar organizer regions enumeration in testicular teratoma

应用银染色技术对1O例睾丸正常组织和13例睾丸良恶性畸胎瘤细胞进行了核仁组成区(NORs)研究,结果发现:正常睾丸及畸胎瘤的核仁组成区嗜银蛋白(AgNORs)颗粒呈圆形,规则,边界清楚,大小均匀,位于胞核编中成边缘部位;畸胎癌的AgNORs颗粒呈圆形或卵圆形,欠规则,边界欠清,大小较不一致,偏中或散在分布于核内.AgNORs计数正常组为1.56±0.17,畸胎瘤组为2.4O±0.26,畸胎癌组为5.24±0.36.各组间有显著性差(P<0.01).AgNORs计数在良恶性畸胎瘤间有分离.认为AgNORs技术对睾丸良恶性畸胎的鉴别是有意义的.

The value of AgNORs enumeration has been investigated in 10 normal testis tissues , 6 benign teratomas and 7 malignant teratomas. The major findings were: the shape of the AgNORs in normal and benign teratoma was round, regular, with clear boundary and even size, setting in the center or the margin of the nucleus; in the malignant teratoma, it's irregular with unclear boundary and uneven size, setting in the near center or scattered in the nucleus. AgNORs enumeration: normal group: 1. 56 ±0. 17 ,benign teratoma: 2. 40±0. 26,malignant teratoma : 5. 24±0. 36. Three was significant difference between each two group ( P <0. 01), the range of AgNORs enumeration in benign and malignant teratoma was separat. These results indicate the ma thod is helpfull in differentiation the benign and malignant testicular teratoma, and may provide us an objective parameter.