导入TNF-αcDNA片断鼠LAK细胞对小鼠移植肿瘤治疗的初步观察

Treatment of Implanted Murine Tumor by Murine LAK Cells Transduced with TNF α cDNA

利用逆转录病毒中介的基因转移技术，将重组人ＴＮＦ－αｃＤＮＡ功能片断导入小鼠脾ＬＡＫ细胞中，然后用此细胞对荷瘤小鼠局部治疗，并以导入ＮｅｏＲ基因的ＬＡＫ细胞做对照。结果，经导有ＴＮＦ－α基因的ＬＡＫ细胞治疗的小鼠肿瘤明显变小，甚至消失，小鼠寿命亦明显高于对照组。

Using retrovirus mediated gene transfer technique, we transduced the recombinant TNF α cDNA into murine LAK cells. With these transduced LAK cells, we treated the tumor bearing mice implanted by S180 cells.The results were:(1)The tumor of the mice treated with TNF α transduced LAK cells became obviously smaller or even disapeared; (2)The life span of the mice treated with TNF α trnsduced LAK cells was much longer than that of those traeated with Neo R gene transduced LAK cells or with N S. These demonstrated that the transduction of TNF α gene into mice could convey an obvious antitumor effect in vivo, and testified the feasibility for clinical application of TNF α gene therapy for cancer. [WT5”HZ〗