双胆囊培植牛黄的作用研究

Studies on Cultural Calculus Bovis in Double Gallbladder

选用健康黄牛28头，施行双胆囊植黄手术，使牛黄同时在模拟胆囊和胆囊内牛黄床上生长，并分别测定了植黄前、手术后3、7、15、30、60d的牛血清和胆汁成分。结果表明：手术后3d的胆汁颜色由墨绿色变成黄绿色；胆汁pH值、干重、比重、粘度、胆酸、粘蛋白的含量也都显著下降；体内实验中模拟明器的Ca2+浓度无明显变化。采用向牛体内的模拟胆囊注入大肠杆菌、肝泰乐来提高胆红素的转化率，加入Ca2+促进胆红素沉淀，月产牛黄6．80±1．80g，胆红素含量达到或超过35％的占61．54％，实验组胆红素含量明显高于对照组（P＜0.05），结果表明双胆囊培植牛黄效果优于模拟胆囊和胆囊内培植牛黄。

28 healthy cattle Were used to receive double gallbladder cultural calculus bovis (CCB) surgical operation. The bile in gallbladder flowed into artificial gallbladder (AG) then duodenum, and the CCB growing on the calcuIus bovis bed in the AG and gallbIadder. It showed that the bile's color changed from blackish grcen to yellowish green after 3 days of operation, and the pH, dry weight, density, riscosity, cholic acid and mucin of the bile decreased obviously. E. coi and glucurolactone were injected into AG to improve the invert rate of bilirubin and Ca2+ were used to promote the settle ment of bilirubin. The production was 6. 8 ±1. 80 g/mo, and 61. 54 % of cultural calculus bovis'bilirubin concent was more than 35％. The content of bilirubin in expcrimcnt group was higher than that in control group (P<0. 05). It suggests that CCB in DG was effective than in the AG and gallbladder.