神经生长因子对脊髓前角运动神经元的保护作用

Protective effect of nerve growth factor on spinal motor neurons

目的：证实神经生长因子（ｎｅｒｖｅｇｒｏｗｔｈｆａｃｔｏｒ，ＮＧＦ）对脊髓前角运动神经元有保护作用。方法：切断ＳＤ大鼠一侧坐骨神经，借助单盲端硅胶管系统在神经损伤局部给予ＮＧＦ。术后行酶组织化学检测。结果：坐骨神经切断可致腰段脊髓前角运动神经元明显损害，其表现为神经元乙酰胆碱脂酶（ａｃｅｔｙｌ－ｃｈｏｌｉｎｅｓｔｅｒａｓｅ，ＡｃｈＥ）活性降低和酸性磷酸酶（ａｃｉｄｐｈｏｓｐｈａｔａｓｅ，ＡＣＰ）活性升高，应用ＮＧＦ可显著改善上述酶学变化。

Objective:To investigate the protective effect of nerve growth factor(NGF) on spinal motor neurons. Methods: The sciatic nerve was sectioned unilaterally and NGF was supplied locally to the proximal nerve stump using a single blind silicone tube. Enzyme histochemical technique was conducted after operation. Results: Sciatic nerve section did injure the spinal motor neurons. There was a decrease in acetylcholinesterase(ACHE) activity and an increase in acid phosphatase(ACP) activity. Application of NGF could significantly improve the above mentioned enzymatic activity changes. Conclusions: NGF plays a role in protecting the spinal motor neuron against injury.