摘要
采用甜瓜蔓枯病病菌孢子悬浮液对208份甜瓜种质资源进行苗期人工接种鉴定,发现86份材料表现抗病,122份材料表现感病。用RAPD标记对抗性表现最优的29份材料进行分析,14个引物共扩增出116条多态性条带,平均每个引物为8.29条。Shannon多样性指数和Nei's基因多样性指数分别为0.4331和0.2855,表明这些抗源材料遗传差异较大。UPGMA聚类分析将这29份抗性种质明显划为普通型和野生型两大类,种质问还表现出一定的地域特性。
Two hundred and eight accessions of melon germplasm were screened and evaluated for resistance to gummy stem blight（GSB）by artificial inoculation at the seedling stage. The results showed that eighty six among them were resistant and one hundred and twenty two were susceptible. Fourteen RAPD primers were used to analyze the genetic diversity of the twenty nine accessions with the most GSB resistance. In total one hundred and sixteen DNA polymorphic bands were scored, with an average of 8.29 bands for each primer. Shannon＇s information index（Ⅰ） and Nei＇s gene diversity index were 0.4331 and 0.2855, respectively, indicating rather large genetic distances among those germplasm. Cluster analysis by UPGMA divided these twenty nine accessions into two groups, and the accessions with the same geographical origin were clustered together.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第5期1201-1206,共6页
Acta Horticulturae Sinica
基金
国家高技术研究发展计划专项经费资助项目(2004AA241170)
江苏省自然科学基金项目(BK2005088)
关键词
甜瓜
蔓枯病
抗性
RAPD
遗传多样性
Melon
Gummy stem blight
Resistance
RAPD
Genetic diversity
