逼尿肌不稳定大鼠逼尿肌组织三磷酸肌醇受体变化的研究

The study on changes of detrusor tissue inositol (1,4,5)-trisphosphate receptors in detrusor instability rats

目的：探讨逼尿肌不稳定（DI）大鼠逼尿肌组织三磷酸肌醇受体（IP3R）变化与DI的关系。方法：建立DI大鼠模型,应用逆转录-聚合酶链反应（RT-PCR）方法检测DI及逼尿肌稳定（DS）大鼠逼尿肌组织IP3R亚型变化。结果：DS大鼠及DI大鼠膀胱逼尿肌组织均有IP3RⅠ、Ⅱ、Ⅲ亚型表达,DI大鼠IP3RⅠ、Ⅱ亚型表达显著高于DS大鼠（P〈0.05-0.01）,IP3RⅢ亚型表达差异无统计学意义（P〉0.05）。结论：DI大鼠IP3RⅠ、Ⅱ亚型表达增强可能参与了逼尿肌不稳定的发生。

Objective： To investigate the relationship between the changes of detrusor tissue inositol（1,4,5）- trisphosphate receptors （IP3 R） in detrusor instability（DI） rats and detrusor instability （DI）. Methods： To set up the model of Di rats, the IP3R isoforms expressions of detrusor tissue in DI rats and DS rats were detected by RT-PCR technique. Results： Three subtypes of IP3R expressed in DI and DS rats detrusor tissue were identified. The expression of IP3 R - Ⅰand IP3 R- Ⅱ in DI rats were significantly higher than those of DS rats （P 〈0.05-0.01）. The expression of IP3R-Ⅲ didn＇t have significant difference between DI and DS rats （P 〈0.05）. Conclusions： The increment of IP3R Ⅰ and Ⅱexpression in DI rats might contribute to the pathological mechanism underlying DI formation.