HPV16/18、p53在新疆贲门腺癌中的表达及其病理生物学意义

Biopathological significance of the abnormal expression of HPV16/18、p53 in cardiac cancer tissues in Xinjiang

目的:检测新疆不同民族贲门癌与非癌良性组织中HPV16/18与p53的异常表达是否存在差异,并讨论其意义。方法:应用PCR及免疫组化LSAB法检测93例贲门腺癌和21例非癌组织的HPV16/18、p53的阳性检出率。结果:(1)HPV16/18、p53在93例贲门腺癌中的检出率分别为34.41%(32/93)、75.27%(70/93),在贲门非癌良性组织中的阳性检出率分别为0%(0/21)、4.76%(1/21)。它们的阳性率在癌和非癌组织间均存在统计学差异(P<0.01)。(2)哈萨克族、维吾尔族、汉族之间HPV16/18、p53阳性率的差别均无统计学意义(P>0.05)。(3)p53的阳性表达与组织分级无关(P>0.05),而与淋巴结转移相关(P<0.05)。HPV16/18的阳性表达与贲门腺癌组织学分级及淋巴结转移无明显关系(P>0.05)。(4)贲门腺癌中HPV16/18与p53间未发现有相关关系(P>0.05)。结论:(1)HPV16/18蛋白和p53的异常表达在贲门癌变过程中具有重要作用。(2)贲门癌组织中HPV16/18、p53表达在肿瘤分化程度、民族之间未见统计学差别。(3)p53的阳性表达与淋巴结转移相关(P<0.05)。

Objective： To detect the abnormal expression of human papillomavirus（HPV16/18）, p53 oncogene in cardiac cancer tissues in different ethnic patients, may involve the molecular biology of cardiac carcinogenesis. Methods： The over expression of HPV16/18 and p53 on paraffin embedded tumor tissues in 93 different ethnic patients with cardinal Adenocarcinoma （Hazak30, Uygur32, Han31） were examined by immunohistochemistry （LSAB） and the polymerase chain reaction （PCR） distinctively. Results. The positive frequency of HPV16/18 and p53 in cardial cancer were 34.41%（32/93）, 75.27%（70/93）respectively. And the expression of those in non-cancer was 0%（0/21）, 4. 76%（1/21）. There were statistic significance between cancer and non-cancer （P 〈0.01）. The statistic significance was found between the expression of p53 and the metastasis in lymph node （P 〈0.05）. However, no difference of the expression of HPV16/ 18, p53 among different ethnic as well as varing degrees of differentiation was found （P 〉0.05）. No significant correlationship was found between HPV16/18 and p53 （P 〉0.05）. Conclusion： The abnormal expression of HPV16/18 and p53 may be an important etiopathogenesis factor for cardiac carcinogenesis. No statistic difference on the expression of HPV16/18 and p53 among different ethnic was found as well as varing degrees of differentiation. The abnormal expression of p53 had relationship with matestasis of lymph node incardiac cancer.