急性肺损伤对大鼠脾脏SOD、MDA、一氧化氮及结构的影响

The changes of SOD, MDA, NO and structure in spleen of rats during ALI

目的:研究急性肺损伤(acute lung injury,ALI)对大鼠脾脏的丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutases,SOD)、一氧化氮(nitrogen monoxidum,NO)及结构的影响。方法:16只Wister大鼠随机分为对照组(C组)和油酸组(OA组),后者由尾静脉注射油酸法复制ALI模型2h后,测定血气(pH、PaO2、MAP、PaCO2)、肺的湿/干重(W/D)及血浆和脾脏匀浆SOD、MDA、NO。脾脏组织做光、电镜检查。结果:与C组相比,OA组PaO2、MAP降低(P<0.01);血浆和脾脏的SOD活性降低(P<0.01),MDA和NO含量增高(P<0.01);光镜下OA组脾脏被膜增厚,鞘淋巴细胞增生,灶状中性白细胞浸润,生发中心异常和红髓吞噬含铁血红素细胞增多改变;电镜下细胞肿大,核膜皱缩,核大畸形,线粒体水肿,空泡变和粗面内质网扩张改变,排列紊乱。结论:ALI可引起大鼠脾脏SOD、MDA、NO及结构的改变,氧化应激反应导致脂质过氧化损伤可能是ALI时远隔器官受损的原因之一。

Objective： To investigate the changes of SOD, MDA ,NO and structure in spleen of rats during ALL Methods： Sixteen Wistar rats were randomly divided into control group （C group） and experimental group （OA group）, pH, PaO2 ,MAP, PaCO2 and pulmonary W/D were measured. Indexs of SOD MDA and NO of plasma and spleen were assessed. The structure of spleen was observed by both light microscope and transmission electronmicroscope. Results： Compared with C group, PaO2 and MAP of OA groups reduced （P 〈0.01）; SOD activity in plasma and spleen of OA group was lower than that of C group （P 〈0.01） ; Levels of MDA and NO in plasma and spleen of OA groups increased （P 〈0.01）. Caps of spleen thickening, leukomonocyte proliferation, neutrophilic infiltrating, germinal center enlargement and red pulp phagotrophic cytosis in OA can be seen by light degeneration, endoplasmic reticulum dilatation. Conclusions： ALI can caused changes of SOD, MDA, NO and destroyed structure of spleen. Lipid peroxidation oxidative damage resulting from stress reaction may play roles in spleen injury during ALI.