新疆哈萨克族ANP基因T1766C多态性与原发性高血压的关联性

Association of the T1766C polymorphism of atrial natriuretic peptide (ANP) gene with essential hypertension in Xinjiang Kazakh

目的:探讨哈萨克族人群心房利尿钠肽(ANP)基因T1766C多态性与原发性高血压的关联性。方法:用聚合酶链反应、限制性内切酶的方法检测新疆塔城地区哈萨克族247名原发性高血压患者(高血压组)和245名正常人群(对照组)T1766C基因多态性。结果:哈萨克族正常人群及高血压患者的T1766C基因多态CC、TT、TC基因型频率分布分别为0.025、0.119、0.857和0.020、0.199、0.780,C和T等位基因分布频率分别为0.453、0.547和0.411、0.589,符合Hardy-Weinberg平衡。分析结果表明,高血压组及对照组ANP基因T1766C的C及T等位基因分布差异无显著性(χ2=1.787,P=0.181),基因型频率差异无显著性(χ2=5.932,P=0.052)。结论:ANP基因T1766C多态性与新疆塔城地区哈萨克族原发性高血压无关联。

Objective： To investigate the association of the T1766C polymorphism of atrial natriuretic peptide gene with essential hypertension in Xinjiang Kazakh. Methods： The study covered 247 hypertensive cases and 245 norrnotensive controls in Xinjiang Kazakh population. The segment of T1766C was amplified from DNA by polyrnerase chain reaction （PCR）. The PCR products were digested by restriction endonuclease. Result： The frequencies of C and T in hypertensive group （0. 411 and 0. 589） were not significantly different from those in the control group （0. 453 and 0. 547; ;（2 =1. 787, P =0. 181）. The frequencies of T1766C genotypes of CC, TT and TC were 0. 020, 0. 199 and 0. 780 in hypertensive respectively, and 0. 025, 0. 119 and 0. 857 in controls respectively. There was no significant difference in genotype between hypertensive group and norrnotensive group（x^2 =5. 932, P =0. 052）. Conclusion： The result suggests that the T1766C polyrnorphisrn of ANP gene may not be associated with hypertension in Kazakh population of Xinjiang area.