人结肠癌裸鼠原位移植瘤模型的建立

Establishment of Orthotopic Transplantation tumor and metastasis Model of Human Colorectal Tumor in Nude Mice

目的建立人结肠癌裸鼠原位移植瘤模型。方法使用对数生长期的人结肠癌细胞(lovo)在8只裸鼠结肠浆膜至黏膜逐层注射,以完成原位移植瘤模型的制备,同时皮下种植8只裸鼠作为对照组。分别于第4、6、8、12周各组分别处死裸鼠2只,观察原位种植肿瘤的成瘤率、生长情况、转移率和腹水出现率。结果16只裸鼠实验期间无1只死亡,成瘤率为100%,原位种植成瘤率为100%(8/8),区域淋巴结转移率100%(8/8),肝转移率为100%(8/8),肺脏转移率为75.0%(6/8),腹膜转移率为75.0%(6/8),腹水出现率为27.5%(3/8)。皮下种植组未见转移。结论本实验成功建立了人结肠癌裸鼠原位移植瘤模型,该模型的生物学行为与临床病程非常相似,为研究人结肠癌转移机制和干预措施提供了较为理想的动物模型。

Objective To establish an orthotopic transplantation model of human colorectal tumor in nude mice. Methods The cell line LOVO in log phase of human colorectal adenocarcinoma was harvested and orthotopically implanted in the cecum subserosa of 8 nude to develop implant-tumor. Simultaneously , to implanted subcutaneously in 8 nude mice as control group. To respectively sacrifice two nude mice of two groups every time at 4,6,8,12 weeks later, Then the formation of implanted tumor rate, local tumor growth characteristics,and metastasis rates were examined. Results None of the nude miee died,all nude mice formed transplantation neoplasma. In insitu transplantation group,The incidences of local lymphatic metastasis, liver, lung, and peritoneal metastases were 100%,100% ,75.0% and 75.0% respectively. The incidences of aseites was 27.5%（3/8）. In contrast,there was no one metastasis in implanted subcutaneously group. Conclusion The successful establishment of orthotopie transplantation model of human colonic tumor in nude mice. The orthotopie implantation tumor and metastasis model provide useful tools for the study of mechanism of metastasis and its treatment of human colon cancer.