结缔组织生长因子转染对人乳腺癌细胞系生物学行为的影响

Biological effects of connective tissue growth factor transfection on human breast cancer cells

背景与目的:结缔组织生长因子(CTGF)是CCN家族成员之一,参与体内多种生理和病理生理过程。本研究探讨CTGF对人乳腺癌细胞生物学行为的影响。方法:构建含有CTGF开放读码框的真核表达质粒,通过脂质体介导的方法将正义质粒转染MCF-7细胞,反义质粒转染MDA-MB-231细胞。观察CTGF不同表达水平与乳腺癌细胞增殖、凋亡、周期分布和体外侵袭等生物学行为的关系。结果:转染正义质粒使MCF-7细胞CTGF表达上调,抑制细胞增殖,促进细胞凋亡,细胞发生G0G1期阻滞,体外侵袭能力下降。转染反义质粒使MDA-MB-231细胞CTGF表达下调,促进细胞增殖,减少细胞凋亡,增强细胞体外侵袭能力,但对细胞周期无影响。结论:CTGF具有抑制乳腺癌细胞生长和侵袭的功能。促进细胞凋亡是CTGF抑制乳腺癌细胞体外生长的机制之一。CTGF可能对细胞周期有一定的影响。

Background and purpose： Connective tissue growth factor（CTGF） is a member of CCN family, it has been reported that CTGF involve in many biological processes and various pathological conditions. In our study, the correlation of CTGF expression and biological effects on breast cancer cell lines were investigated. Methods： The eukaryotic expression vectors containing CTGF open reading frame（ORF） pcDNA3.0/CTGF were constructed and transfected into breast cancer cell lines. The relationship between CTGF expression and breast cancer cell growth ability and proliferation, cell cycle distribution, apoptosis, cell motility and invasive capacity in vitro were observed. Results： Upregulation of CTGF expression in MCF-7 cell line could inhibit its growth ability and proliferation, increased the proportion of G0G1 phase cells, enhanced apoptosis and inhibited its invasive ability in vitro. Downregulation of CTGF expression in MDA-MB-231 cell line increased its growth ability and proliferation, decreased apoptosis and promoted its invasive ability in vitro. There were no differences of cell motility among different groups in MCF-7 and MDA-MB-231 cell lines. Conclusions： CTGF can inhibit breast cancer cell growth by increasing cell apoptosis and/or the proportion of cells in G0G1 phase. CTGF can also inhibit breast cancer cell invasive ability.