骨桥蛋白在人子宫内膜、早孕蜕膜和绒毛中的表达及意义

Study on expression of osteopontin in human endometrium throughout the menstrual cycle and early pregnant endometrium and chorionic tissues.

目的:通过检测育龄妇女正常月经周期中不同时期的子宫内膜组织、早孕蜕膜和绒毛组织中骨桥蛋白(osteopontin,OPN)的表达,探讨OPN在子宫内膜表达的变化规律及其在胚胎着床过程中的作用。方法:采用免疫组化和逆转录聚合酶链反应等技术,检测正常月经周期的子宫内膜组织、早孕蜕膜、绒毛中骨桥蛋白的表达。结果:OPN在月经周期的各期子宫内膜均有表达,以分泌中期子宫内膜组织的表达最强,且分泌中、晚期的表达量明显高于增生期和分泌早期(P<0.05)。早孕蜕膜和绒毛组织中OPN均呈强表达,明显高于非孕各期子宫内膜组织(P<0.05)。结论:OPN mRNA及其蛋白在分泌中期(种植窗期)子宫内膜的表达明显升高,推测OPN可能与种植窗的开放密切相关。OPNmRNA及其蛋白在早孕蜕膜和绒毛组织中的表达水平进一步升高,提示OPN在胚胎着床后也有重要作用。

Objective ：To investigate the expression of osteopontin in reproductive women′s endometrium throughout the menstrual cycle,and early pregnant endometrium and cho- rionic tissue, and discuss the regularity of osteopontin expression in human endometrium and its role during embryo implantation. Methods： The expression of osteopontin in human endometrial specimen and early pregnant endometrium and chorionic tissues was detected by semi-quantita- tive RT-PCR and immuno-histochemistry. Results： Osteopontin mRNA and protein were ex- pressed in human endometrium throughout the menstrual cycle. The expression levels of os- teopontin mRNA and protein were all significantly higher in human mid and late secretory endo- metrium compared with proliferative endomtrium and early secretory endometrium （ P 〈 0.05 ）. Osteopontin was also expressed in early pregnant endometrrium and chorionic tissues. Moreover, the expressive quantity of osteopontin mRNA and protein were significantly higher in these tis- sue than that in nonpregnant endometrium （P 〈 0.05 ）. Conclusions： The expression of os- teopontin mRNA and protein are increased in human mid secretory endometrium （around the time of “implantation window” ）, indicating that osteopontin may participate in opening “im- plantation window”. Osteopontin mRNA and protein are increased further in early pregnant en- dometrrium and chorionic tissues, presumed that osteopontin may play a critical role during the time of after embryo implantation.