脂肪酸甲酯调控鼠骨髓间质干细胞增殖作用的研究

Effect of Fatty Acids Methyl Ester from Plastrum Testudinis on Proliferation of Rat Bone Mesenchymal Stem Cell

龟板浸膏采用石油醚、乙醚、二氯甲烷依次提取,将提取物进行甲酯化处理。用MTT(商品名为噻唑蓝,化学名为3-(4,5)-2-唑噻-(2,5)-二苯基溴化四氮唑蓝)法及流式细胞仪,研究了甲酯化产物调控鼠骨髓间充质干细胞活性,采用气质联用和高效液相色谱技术研究了龟板浸膏提取物甲酯化产物的化学成分,结果表明,3个甲酯化样品都能促进干细胞增殖,而且都含有十六烷酸甲酯,当十六烷酸甲酯浓度为0.15μg/μL时能促进干细胞增殖,由此初步推断龟板浸膏甲酯化产物促进干细胞增殖与十六烷酸甲酯有关。这为中医药调控干细胞的研究提供重要的参考。

The ointment of plastrum testudinis was extracted by petroleum ether, ether and dichloromethane in turn, to gain the extracts, and the extracts were methyl esterified. The effects on the proliferation of bone marrow mesenchymal stem cells （bMMSCs） were examined by MTF [ 3-（4,5-dimethylthiazol-2-yl）-2,5- diphenyl tetrazolium bromide ] assay and flow cytometry analysis. The volatility components of the samples were studied by gas chromatography-mass spectrometry （GC-MS）and high performance liquid chromatography （HPLC）. The results show that the methyl-esterified parts can promote the proliferation of stem cells, and they all contain palmitic acid methyl ester, palmitic acid methyl ester can promote proliferation when the concentration was 0.15 ×10 -6 μg/μL. It may be concluded that the palmitic acid methyl is important for methylesterified parts that have effects on proliferation. This result provides important reference for stem cells research of Traditional Chinese Medicine.