反相高效液相色谱法测定化妆品中的24种防腐剂

Determination of 24 Preservatives in Cosmetics by Reversed Phase High Performance Liquid Chromatography

建立了同时检测化妆品中24种防腐剂含量的反相高效液相色谱法（RP—HPLC）。采用KromasilC18（4．6mm×250mm，5μm）色谱柱，以磷酸盐缓冲溶液（pH=4．26）为流动相，梯度洗脱。样品经甲醇超声提取，然后采用RP—HPLC-二极管阵列检测法测定，对样品前处理和色谱条件进行研究和优化。方法的相对标准偏差为1．3％～3．3％；回收率为90．6％～97．8％；各种防腐剂的线性相关系数≥0．9997，具有较高的准确度和精密度，可用于化妆品中24种防腐剂含量的分析。

A reversed phase high performance liquid chromatographic （RP-HPLC）method has been developed for the simultaneous determination of twenty-four preservatives in cosmetics. A Kromasil C18 column was used with phosphate buffer solution （ pH = 4.26 ） as the mobile phase under the condition of gradient elution. Preservatives were extracted with methanol by ultrasonic, then analyzed by RP-HPLC-diode array detection. The pre-treatment method of sample and the chromatographic condition of analysis were critically examined. The relative standard deviations（RSD） were 1.3% - 3.3% , recoveries were 90.6% - 97.8% , all the corre- lation coefficients（r） were no less than 0. 9997. The method was precise and accurate, and could be used for analyzing the preservatives in cosmetic products.