脂质体电动色谱法评价阿魏酸与生物膜的相互作用

Evaluation of Ferulic Acid-Biomembrane Interaction by Liposome Electrokinetic Chromatography

利用脂质体与生物膜结构的相似性，将脂质体加入毛细管电泳缓冲溶液中作为假固定相，在数分钟内测定了阿魏酸的脂水分配系数Klw,建立了脂质体电动色谱评价阿魏酸与生物膜相互作用的方法。研究了脂质体中胆固醇的含量、缓冲溶液pH值和缓冲体系对Klw的影响。结果表明，在实验条件范围内（胆固醇含量0～30％，pH值4.0～12．0），胆固醇含量升高，缓冲溶液pH值增大，Klw降低；在不同的缓冲体系中，离子强度越大，Klw越大。脂水分配系数的变化反映了阿魏酸与生物膜相互作用。

A facile method for electrokinetic chromatography （ evaluating acidic drug-biomembrane interaction was established by liposome LEKC ）. LEKC is a capillary electrophoresis technique where liposome is some and biomembrane. Liposome water partition coefficient （ Klw ） of ferulic acid was determined in several minutes. The effect of cholesterol content, buffer pH and buffer composition on Klw was investigated. An increase in cholesterol content （0- 30% ）and pH values （4.0 -12.0） resulted in a lower Klw. In different buffer systems, the greater ionic strength was, the larger KlwWaS. The difference of Klw revealed ferulic acid-biomembrane interaction.