微乳液相色谱法及其应用进展

Microemulsion Liquid Chromatography and Recent Application Progresses

微乳液相色谱法是使用普通的正相或反相色谱柱,分别以油包水或水包油型微乳为流动相,用常规检测器进行样品分析的液相色谱法。该法具有独特的选择性,能够同时分离极性范围很广的化合物,流动相中的有机溶剂用量少,调节参数多,以及血浆样品可直接进样,梯度洗脱节省再平衡时间等优点,在复杂组分分离方面具有显著的优势。本文对微乳液相色谱法中常用微乳的组成和结构,各组成成分对分离的影响,以及该法的应用进展进行了综述。

Microemulsion liquid chromatography （MELC） is defined as a kind of liquid chromatography using microemulsions as mobile phases, in which water-in-oil and oil-in-water microemulsions are used on normal and reverse phase columns, respectively, while the detectors are the same as conventional liquid chromatography. Many specific advantages of MELC have been demonstrated, including unique selectivity, high efficiency, the ability to separate simultaneously compounds covering diverse solubility and to inject directly plasma samples onto the HPLC system and elimination of the need for an equilibration rinse between gradients. This article introduced the composition and physicochemical structures of different microemulsions used in MELC, and discussed the effects of operating parameters on separation in detail. Some applications were illustrated and a promising outlook of MELC was described.