含不同Anti-Waxy基因拷贝数的稻米直链淀粉含量分析

Reduction of Amylose Contents in Transgenic Rice with Different Copies of Anti-Waxy Gene

将含单拷贝Anti-Waxy基因的纯合植株分别作为父本或母本进行正反交,获得两组含双拷贝Anti-Waxy基因的杂交后代,分析了未转基因对照、转基因亲本及两组杂交后代糙米直链淀粉含量以揭示不同Anti-Waxy基因拷贝数对降低稻米直链淀粉含量程度的影响.结果显示,2个单拷贝转基因水稻糙米直链淀粉平均含量分别为10.72%和11.13%,比对照分别降低17.98%和14.84%;两组正交和反交杂交后代糙米直链淀粉平均含量分别为8.96%和8.23%,其平均值为8.60%,比2个转基因杂交亲本糙米直链淀粉含量分别降低19.78%和22.73%,比对照降低了34.20%.结果表明,增加转基因水稻基因组中Anti-Waxy基因拷贝数在一定程度上能够进一步降低稻米直链淀粉含量,通过将独立转化获得同品种转基因植株之间杂交可以成为获得高表达转基因植物的途径.

Experiments were conducted to investigate the reductiong of amylose contents in transgenic rice with different copies of Anti-Waxy. In this study, two different homozygotes with single copy of Anti- Waxy were used as male or female parent to conduct reciprocal crosses,and then two F1 populations with two copies of Anti-Waxy respectively were obtained. We analyzed amylose contents in unpolished rice of the two transgenic parents and the F2 seeds derived from two F1 populations. The results showed that the average amylose contents in the unpolished rice of the two transgenic parents were 10.72% and 11.13% respectively,which was 17.98% and 14.84% lower than that of the wild type; the average amylose contents in the two F2 seeds were 8.96% and 8. 23% respectively, and the mean was 8. 60%, which was 19.78% and 22.73% lower than those of the two transgenic parents,and 34.20% lower than that of the wild type. The results indicated that the more copies of Anti-Waxy there were in the transgenic rice genome, the lower amylose contents were in the seeds. This study provide a new method for obtaining the highly expressed transgenic plants by crossing with the transgenic plants of the same variety.