米非司酮抑制子宫内膜癌细胞体外增殖的作用

Inhibitory Effect of Antiprogestins Mifepristone on the Proliferation of Endometrial Carcinoma HHUA Cell Line in Vitro

目的观察抗孕激素米非司酮对人子宫内膜癌细胞体外增殖活性的影响，并探讨其作用机制。方法体外培养子宫内膜癌HHUA细胞株，不同浓度米非司酮处理细胞24-96h，应用四甲基偶氮唑蓝（MTT）比色法观察米非司酮对HHUA细胞增殖活性的影响；免疫组化技术观察HHUA细胞Ki-67和c-myc基因表达的变化。结果米非司酮以时间一剂量依赖性方式，显著地抑制人子宫内膜癌HHUA细胞的体外增殖活性（P〈0．05）；当米非司酮浓度≥5μmol／L作用细胞24h后，子宫内膜癌HHUA细胞Ki-67和c-myc基因表达水平明显降低。结论抗孕激素米非司酮以时间一剂量依赖性方式显著抑制子宫内膜癌HHUA细胞的体外增殖活性，并与降调Ki-67和c-myc基因表达密切相关。

Objective To investigate the effects and its mechanisms of antiprogestins mifepristone on the proliferation of human endometrial carcinoma cell in vitro. Methods Human endometrial carcinoma HHUA cell were cultured in vitro and treated with mifepristone in different concentration for 24-96 hours. Methyl thiozolyl tetrazolium （MTT） was used to observe the growth suppressive rate of HHUA cell. The expression of Ki-67 and c-myc of HHUA cell were determinded by immunohistochemistry. Re- sults The proliferation of HHUA cell were suppressed with mifepristone in time-dose dependent fashion in vitro （P〈O. 05）. The decreased expression of Ki-67 and c-myc appeared when the mifepristone≥5 μmol/L treated the cell for 24 hours （P〈0. 05）. Coneclusion Antiprogestins mifepristone could signifi- cantly suppress the proliferative activity of human endometrial carcinoma HHUA cell in timedose dependent fashion in vitro. The action mechanisms of mifepristone were closely associated with the decrease of the expression of Ki-67 and c-myc.