单叶蔓荆小孢子发生和雄配子体的发育

Microsporogenesis and Development of Male Gametophyte in Vitex trifolia var.simplicifolia(Verbenaceae)

利用常规石蜡制片法对单叶蔓荆小孢子发生和雄配子体发育进行了详细观察。主要结果如下:(1)花药壁由四层细胞构成,由外到内分别为表皮、药室内壁、中层和绒毡层,花药壁发育方式为双子叶型。(2)花药壁表皮细胞具多细胞腺体。(3)药室内壁和部分药隔细胞具纤维性加厚。(4)绒毡层细胞有两种来源,外周部分来源于初生壁细胞,近药隔部分来源于药隔细胞。绒毡层为分泌型,细胞具双核。(5)小孢子母细胞减数分裂过程中胞质分裂为同时型,形成的四分体主要为四面体型排列,偶有左右对称型。(6)成熟花粉粒为2细胞型,花粉具3孔沟。

The microsporogenesis and development lia were observed by means of conventional paraffin of male gametophyte of Vitex trifolia var. simplicifosections. The main results are as follows： （ 1 ） Anther wall comprises epidermis, fibrous endothecium, one middle layer and glandular tapetum with twonucleate ceils. The development of anther wall belongs to dicotyledonous type. （2） There are muhicellular glands on the epidermis of anther. （3） Wall thickening occurs in both endothecium and some cells of connective tissue. （4）The tapetum has dual origin： towards outside the tapetum is derived from the primary parietal layer, whereas on the inner side it develops from the ceils of the connective. The tapetum is secretory and two-nucleate. （5） Cytokinesis of microsporocyte meiotic division is of simultaneous, and the tetrads are tetrahedral, occasionally isobilateral. （6） The mature pollen grains are two-celled with three colpates.